AutoSpectral: AF Spike-in Experiment

Published: 24 September 2026| Version 1 | DOI: 10.17632/njb5zkyjp6.1
Contributors:
Oliver Burton,

Description

Unstained "microglia" and "alveolar macrophages" identified by sequential gating on unstained mouse brain or lung samples, used to determine the accuracy of autofluorescence during training and unmixing assignment.

Files

Steps to reproduce

See paper and included R code script.

Institutions

Categories

Flow Cytometry, Spectral Unmixing, Autofluorescence

Funders

Related Links

Article
https://www.biorxiv.org/content/10.1101/2025.10.27.684855v1.full
is related to this dataset
Software
https://github.com/DrCytometer/AutoSpectral
is related to this dataset

Licence