Raw and processed data for Titanium nanotopography enhances mechano-response of osteocyte three-dimensional network toward osteoblast activation
Description
Includes all raw and analyzed data corresponding to the publication “Titanium nanotopography enhances mechano-response of osteocyte three-dimensional network toward osteoblast activation”, Material Today Bio (2024). Data cover imaging, molecular quantification, and statistical outputs. https://doi.org/10.1016/j.bioadv.2024.213939
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Nanostructured titanium substrates were fabricated, and MLO-Y4 osteocyte-like cells were cultured on them. Confocal microscopy was performed to observe cellular morphology. Quantitative gene expression analysis (qPCR), PGE₂ measurement by ELISA, and Cx43 detection by Western blot were conducted. Image analysis was performed using ImageJ, and statistical analysis was carried out using Microsoft Excel. All analysis parameters and measurement units are provided in the corresponding data files.
Institutions
- Tohoku Daigaku Daigakuin Shigaku Kenkyuka Shigakubu