Data on the regrowth of cryopreserved E. multilocularis larvae in cotton rats and their infectivity to dogs
Description
This dataset presents information on the long-term storage of Echinococcus multilocularis (Alaskan lineage) in liquid nitrogen. In the current study, data on the cryopreservation of protoscoleces and cyst residues from cotton rats (primary hydatid cysts) experimentally infected with parasitic eggs were collected. Specifically, cotton rats were inoculated with protoscoleces and cyst residues that were mixed with sperm cryopreservation solution and frozen in liquid nitrogen for one month. The data were analyzed based on the proliferation of the parasites and the formation of protoscoleces, and experiments were conducted independently on three separate occasions. Similar to previous reports, the proliferation of frozen protoscoleces and cyst residues was confirmed in all three experiments conducted in cotton rats. Using the method employed in this study, more than 700,000 protoscoleces formed in two cotton rats four months after inoculation with frozen protoscoleces. Furthermore, the recovered protoscoleces were orally administered to dogs and E. multilocularis eggs were detected in their feces. These data demonstrated that cryopreserved E. multilocularis can be restored to its original state. Given the large number of strains identified to date, the methods described in this study will allow for the simple maintenance of strains with minimal animal use and reduced costs.