Inhibition of mTORC1 reduces fibrotic gene expression

Published: 18 October 2023| Version 1 | DOI: 10.17632/nwt9frbd7h.1
Contributors:
,
,
,
,
,
,

Description

Organism: House mouse Experiment type: Expression profiling by high throughput sequencing Summary CONTEXT: Epididymal adipose tissue (EAT) transcriptome studies provide holistic pictures of ob/ob mice with or without rapamycin. OBJECTIVE: To implement EAT gene expression profiling and investigate the link between changes in AT gene expression of ob/ob mice with or without rapamycin. RESULTS: Rapamycin, the inhibitor of mTORC1, reduces fibrotic gene expression.

Files

Steps to reproduce

Ten-week-old male homozygous ob/ob mice were randomly divided into two groups after one week of adaptive feeding. The ob/ob mice in the rapamycin group were intraperitoneally injected with rapamycin (0.5 mg/kg/day) for two weeks. The vehicle group was given the same volume of normal saline injection. After two weeks, the mice were killed, and epididymal adipose tissue (EAT) was taken.

Institutions

  • Shanghai University of Traditional Chinese Medicine

Categories

Endocrinology, Obesity, Adipose Tissue, Fibrosis

Funders

  • Pudong New Area Health Commission clinical characteristic discipline construction project
    Grant ID: PWYts2021-13
  • Pudong New Area Science and Technology Development Fund
    Grant ID: PKJ2022-Y10, PKJ2020-Y19
  • Pudong New Area Health Commission leading talent training program
    Grant ID: PWR12021-06
  • a project funded by the Shanghai Municipal Health Commission
    Grant ID: No. 20204Y0200

Licence