Inhibition of mTORC1 reduces fibrotic gene expression

Published: 18 October 2023| Version 1 | DOI: 10.17632/nwt9frbd7h.1
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Description

Organism: House mouse Experiment type: Expression profiling by high throughput sequencing Summary CONTEXT: Epididymal adipose tissue (EAT) transcriptome studies provide holistic pictures of ob/ob mice with or without rapamycin. OBJECTIVE: To implement EAT gene expression profiling and investigate the link between changes in AT gene expression of ob/ob mice with or without rapamycin. RESULTS: Rapamycin, the inhibitor of mTORC1, reduces fibrotic gene expression.

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Steps to reproduce

Ten-week-old male homozygous ob/ob mice were randomly divided into two groups after one week of adaptive feeding. The ob/ob mice in the rapamycin group were intraperitoneally injected with rapamycin (0.5 mg/kg/day) for two weeks. The vehicle group was given the same volume of normal saline injection. After two weeks, the mice were killed, and epididymal adipose tissue (EAT) was taken.

Institutions

Shanghai University of Traditional Chinese Medicine

Categories

Endocrinology, Obesity, Adipose Tissue, Fibrosis

Funders

  • Pudong New Area Health Commission clinical characteristic discipline construction project
    Grant ID: PWYts2021-13
  • Pudong New Area Science and Technology Development Fund
    Grant ID: PKJ2022-Y10, PKJ2020-Y19
  • Pudong New Area Health Commission leading talent training program
    Grant ID: PWR12021-06
  • a project funded by the Shanghai Municipal Health Commission
    Grant ID: No. 20204Y0200

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