Immunoblots for data in Qin et al, 2025, SARS-CoV-2 enhances lysosomal exocytosis efficiency and deacidifies lysosomes to facilitate viral release
Description
Page 2 presents the original, unedited images to generate Figure S1E. Effects of CID1067700 on intracellular LAMP1 in SARS-CoV-2-trVLP-infected cells (8–14 h pi) by western blot. DMSO treatment served as negative control. The samples of the immunoblots that were shown in Figure S1E are highlighted by the red boxes. Page 3 presents the original, unedited images to generate Figure 2C.The effect of SARS-CoV-2 trVLP infection on the total LAMP1 level. Representative immunoblot analysis of LAMP1 and actin in cell lysate of uninfected and SARS-CoV-2- trVLP-infected Caco-2-N cells are shown. Page 4 presents the original, unedited images to generate Figure 2E. The effects of SARS-CoV-2 trVLP infection on the release of cathepsin D and cathepsin L. Representative immunoblot analyses of lysosomal cathepsin D and cathepsin L released into the culture medium of uninfected and SARS-CoV-2- trVLP-infected cells are shown. Page 5 presents the original, unedited images to generate Figure S2A. Quantitative analysis of cathepsin D and cathepsin L in uninfected and SARS-CoV-2-trVLP-infected Caco-2-N cells. Representative immunoblot analyses of intracellular lysosomal cathepsin D and cathepsin L of uninfected and SARS-CoV-2-trVLP-infected cells are shown. The samples of the immunoblots that were shown in Figure S2A are highlighted by the red boxes.