Single-cell and spatial transcriptomics reveal cellular dynamics in the regenerating heart

Published: 15 September 2025| Version 4 | DOI: 10.17632/p38cyb98tc.4
Contributors:
Lei Fan, Qi Tang, Yutong Wang, Hang Sun, Ge Li, Yi Yang, zhaoyuan liu, Hongyan Wang, Qing Jing, Christopher L. Antos, Hui Xiao, Renchu Guan, Gang Pei, Florent Ginhoux, Zhigang Zhou, An Zeng

Description

In neonatal mice, the heart possesses a robust regenerative capacity following injury, which is rapidly lost by postnatal day 8 and in adulthood. In this study, we performed an integrated analysis of the regenerating heart during the neonatal stage using single-cell and spatial transcriptomics. We developed and applied a novel algorithm, SDIMGO, to reconstruct spatial transcriptomic profiles at single-cell resolution. By delineating spatial domains, we identified functionally coordinated cellular ensembles, uncovering key spatial organizational features and cooperative mechanisms involved in cardiac regeneration.

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Institutions

  • Chinese Academy of Sciences

Categories

Immunity, Cardiac Regeneration

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