Applied soil Ecology data
Description
a better understanding of biochar–salinity interactions is needed to determine how feedstock-specific biochars regulate microbial community structure, diversity, and ecological functioning under different levels of salinity stress. To overcome these knowledge gaps, a pot experiment was conducted under paddy field conditions for the study of the effect of different salinity levels of onion peel, garlic peel, ginger peel and clover leaves biochars. The goals were to: (1) assess the effects of biochar–salinity interactions on soil and plant responses; (2) understand the shifts in soil bacterial and fungal community structure and diversity in response to different biochar–salinity treatments; and (3) elucidate the relationships between soil physicochemical properties and microbial community structure under the combined effects of biochar amendment and salinity stress. After 70 days, vegetative growth measurements were conducted to evaluate how treatments affected plant growth. For each pot number of tillers were counted manually. Plant height was measured from the soil surface to the flag leaf tip with a meter ruler. The plant fresh weight (PFW; g pot─1) was measured at harvest and the biomass oven dried at 105 °C for 48 h until constant weight was reached. After drying, plant dry weight (PDW; g pot─1) was determined by weighing the dried biomass. Under full sun (10:00 a.m. to 2:00 p.m.), the chlorophyll content was measured using a SPAD meter (SPAD-502, Minolta Co., Ltd., Japan). Three fully expanded mature leaves were sequentially measured from the top to the bottom of the representative plant that was randomly selected from each replicate. Upon completion of the experiment, soil samples were obtained from each pot and separated into two portions. Soil was split into two parts: one was kept moist for microbial analysis and the other was air dried at room temperature, ground to a fine powder, sieved through a 100-mesh screen and stored for later determination of soil physicochemical properties. The total genomic DNA of rhizosphere soils was isolated using TGuide S96 Magnetic Soil/Stool DNA Kit. The DNA isolated from these cells was quantified using NanoDrop spectrophotometry and analysed for quality by agarose gel electrophoresis. 16S rRNA gene V3–V4 hypervariable region was amplified using 338F (5′-ACTCCTACGGGAGGCAGCA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) primers which contained Illumina adapter sequences and unique index barcodes to facilitate multiplex sequencing. Paired-end sequencing was carried out using the PCR products after optimization of PCR conditions and purification and quantification.
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Institutions
- Hainan UniversityHainan, Haikou