Data from: Ratcheted unidirectional transport and sequential assembly of the yeast telomerase RNP
Description
This dataset includes the original images for Spot tests, Western blots, Southern blot and In vitro telomerase assays, as well as the raw data for qPCR and smFISH analyses used in the paper ''Ratcheted unidirectional transport and sequential assembly of the yeast telomerase RNP''. The telomerase ribonucleoprotein particle (RNP) replenishes telomeric DNA and minimally requires an RNA component and a catalytic protein subunit. However, telomerase RNP maturation is an intricate process occurring in several subcellular compartments and is incompletely understood. Here, we report how the co-transcriptional association of key telomerase components and nuclear export factors leads to an export-competent, but inactive, RNP. Export is dependent on the 5’-cap, the 3’-extension of unprocessed telomerase RNA, and protein associations. Once the RNP reaches the cytoplasm, an extensive protein swap occurs, the RNA is trimmed to its mature length and the essential catalytic Est2 protein joins the RNP. This mature and active complex is then reimported into the nucleus as its final destination and last processing steps. The irreversible processing events on the RNA thus support a ratchet-type model of telomerase maturation, with only a single nucleo-cytoplasmic cycle that is essential for the assembly of mature telomerase.