Western-blot source data, reagent inventories, and figure source data supporting: Aqueous Hellenia speciosa rhizome extract attenuates hepatic injury in high-fat diet/streptozotocin-induced diabetic rats
Description
This dataset provides the source-data files underlying the figures and the reagent inventories of the study "Aqueous Hellenia speciosa rhizome extract attenuates hepatic injury in high-fat diet/streptozotocin-induced diabetic rats" (Filfilan & Elbeeh). Included: (1) Figure 6 per-field morphometric source values (steatotic-vacuole area, inflammatory-foci count, collagen area, DAB-positive area), each value corresponding to the field displayed in the figure panel; (2) full Western-blot source data underlying Figure 4 - cropped blot strips for 12 protein targets plus loading controls (beta-actin, Lamin B1), ROI/QC overlays, per-target densitometry with per-replicate values, means, SD/SEM and fold-change versus normal control, the composite Figure 4 in TIF/PNG/PDF/SVG, and full processing logs; (3) reagent inventories - the antibody panel with catalogue numbers and RRIDs, and the TaqMan gene-expression assay list. Animals: male Wistar rats (n = 10 per group; six groups). Diabetes was induced by four weeks of high-fat diet followed by a single low-dose streptozotocin injection (35 mg/kg i.p.). Treatments were given orally once daily for six weeks: aqueous Hellenia speciosa rhizome extract (CS-AE, 500 mg/kg/day), metformin (200 mg/kg/day), or their combination. Ethics approval: HAPO-02-K-012-2025-05-2711 (Biomedical Research Ethics Committee, Umm Al-Qura University). Reporting follows ARRIVE 2.0. Not included in this version: per-animal raw values, histopathology photomicrographs, HPLC-DAD chromatograms, and statistical-analysis outputs. These will be added in a later version once finalised from the laboratory primary records.
Files
Steps to reproduce
Western-blot source data: membranes were imaged on a ChemiDoc system. Band regions of interest were defined per target against the corresponding loading control on the same membrane (beta-actin for cytoplasmic targets, total AMPKalpha for the phospho-AMPKalpha ratio, Lamin B1 for nuclear Nrf2). Densitometry was performed with rolling-disc background subtraction. Per-lane profiles, ROI definitions and processing logs are included so that every reported value can be traced back to the raw strip. Values are reported both as within-blot ratios and as fold-change versus the normal-control mean. Figure 6 source data: a single fixed central field of identical size (0.0533 mm2; 40x objective, 4.04 px/um) was cropped from every source image and white-point normalised by one common rule. Morphometry was performed in ImageJ using Ruifrok-Johnston colour deconvolution with one fixed threshold applied identically to every field (steatotic-vacuole area and inflammatory-foci count on H&E; collagen area on Masson trichrome; DAB-positive area on immunostains). These are single-field descriptive measurements, not group means; no inferential statistics are attached to them.