Whole-genome characterization and pathogenicity of novel human-porcine reassortant rotavirus strains G9P[7] and G1P[7] in China

Description

Fig 1.Identification of Porcine Group A Rotavirus (PoRVA) Isolates XXW2023 and HD2023. Fig 2.Phylogenetic analysis of 22 genome segments from representative strains, including XXW2023(G9P[7]) and HD2023(G1P[7]). Neighbor-joining (NJ) trees were inferred in MEGA X (only values above 70% are provided). Fig 3. Nucleotide identity analysis of the 22 genomic segments from representative strains, including XXW2023 (G9P[7]) and HD2023 (G1P[7]). Pairwise nucleotide identity was calculated with SDTv. Fig 4. Cross-over regions in the genomes of XXW2023 and HD2023 identified by RDP4 and SimPlot (Red lines denote the major parent and blue lines the minor parent). Fig 5. Pathogenicity of porcine rotavirus A (PoRVA) strains XXW2023 (G9P[7]) and HD2023 (G1P[7]) in 7-day-old suckling mice. Pups were orally inoculated with 100 μL XXW2023, 100 μL HD2023, or 100 μL DMEM (control) (n=4 in each group). Fig 6. Pathogenicity of porcine rotavirus A (PoRVA) strains XXW2023 (G9P[7]) and HD2023 (G1P[7]) in 1-day-old colostrum-deprived piglets. Colostrum-deprived, 1-day-old piglets were orally inoculated with 4 mL XXW2023 (G9P[7]) or HD2023 (G1P[7]); controls were orally given 4 mL DMEM (n = 6 in each challenge group; n = 3 in control group). Fig 7. Characterization of tissue tropism in porcine rotavirus A.

Institutions

• Nanjing Agricultural University

  Jiangsu, Nanjing

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