Involvement of N6-Methyladenosine modifications of long noncoding RNAs in systemic lupus erythematosus: Datasets and Supplementary Materials
Description
Figure 1. Outlines of the SLE m6A methylation. (a) m6A peaks density in patients with SLE and controls. (b) a number of common m6A peaks in patients with SLE and controls. (c) enrichment of m6A peaks along transcripts, each transcript is divided into 5′UTR, CDS and 3′UTR. (d) sequence motif of m6A-containing peak regions. e, heat map of differentially expressed lncRNAs and mRNAs. f, examples of m6A related-lncRNAs peaks. SLE, systemic lupus erythematosus; HC, controls. Figure 2. The expression levels of the m6A-related lncRNAs in patients with SLE and controls. (a) comparison of linc01410 expression between SLE patients and HC; (b) a lower level of Xist in SLE patients compared with HC; (c) a higher level of linc02446 in SLE patients compared with HC; (d) PSMB8-AS1 expression was decreased in SLE patients. SLE, systemic lupus erythematosus; HC, controls. Figure 3 The co-expressed networks of m6A related-lncRNAs and mRNAs. (a) the lincRNA-mRNA co-expression network of linc02446; (b) the lincRNA-mRNA co-expression analysis of PSMB8-AS1. Figure 4. Schematic depicting the mechanisms of m6A methylation modifications to lncRNAs. ‘Writers’, methyltransferases; ‘Erasers’, demethyltransferases; ‘readers’, m6A-binding proteins Table 1 Associations between the expression levels of m6A related lncRNAs and clinical parameters of patients with SLE P value was obtained through t-test or nonparametric Mann-Whitney U test based on data types. Table 2 Correlations between the expression levels of m6A related lncRNAs and quantitative clinical parameters of patients with SLE SLEDAI, the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K); C3, Complement C3; C4, Complement C4; ESR, erythrocyte sedimentation rate; CRP, C-reactive protein. Table 3. Influences of main medications on m6A related-lncRNAs expression in patients with SLE P value was obtained through t-test or the nonparametric Mann-Whitney U test based on data types.