Cajanine modulates psoriasiform inflammation
Description
Transcriptomics anaylysis-HaCaT cells were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37 °C in a humidified 5% CO2 atmosphere. To establish a psoriasiform keratinocyte model, cells were stimulated with the M5 cocktail as previously described for psoriasis-related keratinocyte activation. Cells were assigned to four groups for transcriptomic analysis: CON, CAJ, M5, and CAJ+M5. CAJ was added 2 h before M5 (10 ng/ml) stimulation and maintained throughout the experiment. Total RNA was extracted from HaCaT cells in the CON, CAJ, M5, and CAJ+M5 groups using TRIzol according to the manufacturer’s instructions. Transcriptome sequencing and bioinformatic analysis were performed by OE Biotech Co., Ltd. Proteomic analysis and metabolomic analysis-Male BALB/c mice aged 6-8 weeks were kept in animal care facilities under pathogen-free conditions. Mice were randomly assigned to three groups: CON: Vaseline 62.5 mg was topically applied, followed 4 h later by CAJ at 50 mg/kg body weight; IMQ: IMQ 62.5 mg was topically applied, followed 4 h later by vehicle; IMQ+CAJ: IMQ 62.5 mg was topically applied, followed 4 h later by CAJ at 50 mg/kg. Skin tissues from the CON, IMQ, and IMQ+CAJ groups were processed for proteomic analysis using LC-MS/MS platform and untargeted metabolomics using UPLC-MS/MS platform by OE Biotech Co., Ltd.