Gene Targets and Pre-validation results for CRISPR Cas-9 editng of hydrogenase genes in Rhodobacter johrii isolate MAY2

Published: 19 December 2023| Version 1 | DOI: 10.17632/rcx3mcssnx.1
Contributor:
Leo Agustin Barcelo

Description

This dataset contain gene sequences for the small and large sub-unit of uptake hydrogenase found in Rhodobacter johrii obtained through whoke genome sequencing. Moreover, it contains gRNA and primer sequences to target these genes for manipulation. Additionally, this contains in-vitro cleavage assay results pre-validating the gRNA sequence as effective in cleaving the target genes as well as the ability of the primers to amplify the genes.

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Institutions

  • University of the Philippines Los Banos

Categories

Purple Bacteria, CRISPR/Cas9, Genome Editing, Hydrogenase

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