Single cell RNAseq sequencing matrix data of peripheral blood mononuclear cells from patients with primary partial hypopituitarism and healthy control subjects
Description
This project enrolled three patients withprimary partial hypopituitarism pedigree and matched healthy subjects, collected their peripheral blood, isolated PBMC cells, and used them for single-cell RNAseq sequencing. CON_1, CON_2, and CON_3 correspond to the single-cell sequencing matrices of PBMCs from three healthy subjects, while PATIENT_1, PATIENT_2, and PATIENT_3 represent the single-cell sequencing matrices of PBMCs from three patients with dwarfism. Each compressed file contains two folders: filter_matrix and raw_matrix, which are the filtered and unfiltered matrix files generated by comparing the reference genome using Cellranger software. The parameters used in Cellranger software analysis are as follows: fq_dir=/home/data/project/10x/raw/PBMC1 $bin count --id= PBMC1_outs \ --localcores= 8 \ --transcriptome= $db \ --fastqs= $fq_dir \ --sample= PBMC1 \ --localmem=64