Raw data from amniotic fluids collected during different parturition conditions in canine species

Published: 17 July 2026| Version 3 | DOI: 10.17632/rpdpjyddtb.3
Contributors:
Marina Estevam, viviane Codognoto, Renatha Almeida de Araújo, Daniella Armani, Paula Rattes, Renata Barreto, Fabiana Ferreira de Souza, Maricy Apparício

Description

Amniotic fluid is a dynamic biological compartment reflecting fetal physiological status and intrauterine homeostasis. While its proteomic profile is well-documented in humans and other domestic species, the canine amniotic fluid proteome, and its variations across different parturition conditions, remains uncharacterized. This study aimed to characterize and compare the amniotic fluid proteomic profile of term canine fetuses delivered by elective cesarean section (ECS), with meconium-stained fluid (MCS), and dystocic deliveries (DYS). Samples were analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), identifying sixteen differentially abundant proteins. The ECS group showed higher abundances of proteins associated with fetal homeostasis, nutrient transport, and metabolic regulation, including albumin, alpha-fetoprotein, serotransferrin, apolipoprotein E, globin A2, fetuin B, and an Ig-like domain-containing protein. In contrast, dystocic fetuses exhibited marked reductions in these transport proteins alongside decreased structural proteins (collagen, keratins and gelsolin) and increased proteolytic and stress-response markers (Meconium-stained fetuses displayed an intermediate proteomic profile, suggesting adaptive remodeling rather than the severe disruption seen in dystocia). These findings provides the first characterization of the canine amniotic fluid proteome and highlights its potential as a source of biomarkers for fetal condition and perinatal adaptation.

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Multivariate analyses were performed using MetaboAnalyst 5.0. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied, and the variable importance in projection (VIP) scores were calculated to identify the proteins contributing most to group discrimination. PCA was also used to visualize sample clustering based on protein expression profiles. Statistical differences among groups were evaluated by one-way analysis of variance (ANOVA) followed by Fisher's least significant difference (LSD) post hoc test. Functional enrichment and gene ontology analyses were performed using the STRING database. A Venn diagram was generated using Venny 2.1 to illustrate the distribution of identified proteins among the experimental groups.

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Categories

Canine Reproduction, Proteomics Experimental Approach, Amniotic Fluid Index

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