Peptide assisted CRISPR/Cas delivery enables high-throughput genome engineering in animal models
Published: 13 November 2025| Version 2 | DOI: 10.17632/rrkvr8h5tg.2
Contributor:
huang xiong hengDescription
A peptide-assistant RNP delivery for embryo genome editing to make animal models.
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Steps to reproduce
We generated Tyr knockout (KO) mice using the PEACE technique. Tail tissues from the mice were collected, and PCR amplification with adapter addition was performed for next-generation sequencing (NGS) analysis. In parallel, a plasmid containing both the AsCpf1 expression cassette and the crRNA expression cassette was transfected into N2A cells. After 48 hours, the cells were harvested and lysed to extract genomic DNA, followed by PCR with adapter addition and NGS for analysis.
Categories
Protein Delivery, Embryo, Animal Model Systems, Genome Editing, CRISPR-Cas12A