Dataset for A loop-mediated isothermal amplification assay to detect Bacteroidales and assess risk of fecal contamination

Published: 4 November 2022| Version 1 | DOI: 10.17632/rshv4pd2rt.1
Contributors:
,
,
,

Description

We developed a rapid risk-assessment assay for fecal contamination by targeting Bacteroidales using loop-mediated isothermal amplification (LAMP). The assay allows for naked-eye observation of reactions with as few as ~8 copies of Bacteroidales per cm2 of the surface in the field. We evaluated this assay with complex field samples as well as on-site field studies. In this dataset, we've included the raw and processed data files associated with the research project.

Files

Institutions

Purdue University

Categories

Food Contamination, Rapid Diagnostics, Food Product Safety Assessment, Fresh Vegetable, Bacteroides

Licence