Dataset for A loop-mediated isothermal amplification assay to detect Bacteroidales and assess risk of fecal contamination

Published: 4 November 2022| Version 1 | DOI: 10.17632/rshv4pd2rt.1
Contributors:
,
, Aaron Ault,

Description

We developed a rapid risk-assessment assay for fecal contamination by targeting Bacteroidales using loop-mediated isothermal amplification (LAMP). The assay allows for naked-eye observation of reactions with as few as ~8 copies of Bacteroidales per cm2 of the surface in the field. We evaluated this assay with complex field samples as well as on-site field studies. In this dataset, we've included the raw and processed data files associated with the research project.

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Institutions

  • Purdue University

Categories

Food Contamination, Rapid Diagnostics, Food Product Safety Assessment, Fresh Vegetable, Bacteroides

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