Protective role of PASH-1 in CGG repeat-driven RNA and protein toxicity in FXTAS

Description

This study aimed to compare global miRNA expression profiles using miRNA-seq in C. elegans strains carrying single-copy insertions of 0 or 99 CGG repeats driven by a pan-neuronal promoter. To further validate miRNA-related findings, transcriptomic RNA-seq was also performed to assess gene expression differences.

Steps to reproduce

Three micrograms of total RNA were used for miRNA and mRNA library construction. All library preparation, sequencing, and subsequent bioinformatic analyses—including expression profiling, heatmap generation, and volcano plot visualization—were performed by the Taiwan Genomic Industry Alliance Inc., a commercial genomics service provider

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