Gut microbiota and metabolomic profiles in mice with alcoholic liver disease treated with Brassica rapa L. polysaccharides

Description

This dataset contains the processed abundance data for the study of alcoholic liver disease.

Steps to reproduce

1. Animal Model & Treatment: A mouse model of alcoholic liver disease (ALD) was established. The mice were grouped and treated with Brassica rapa L. polysaccharides (BRLP) or served as controls/models. Fecal and serum samples were collected at the end of the intervention. 2. Gut Microbiota Analysis (microbiota_2.xlsx): Total genomic DNA was extracted from fecal samples. The 16S rRNA gene (e.g., V3-V4 region) was amplified and sequenced using the Illumina platform. The raw sequencing data were demultiplexed, quality-filtered, and processed using standard pipelines (such as QIIME 2) to generate the final OTU/ASV abundance table. 3. Untargeted Metabolomics Analysis (metabolomic data.xlsx): Fecal and serum metabolites were extracted using appropriate solvents and analyzed via LC-MS/MS. Raw mass spectrometry data were processed for peak alignment, peak picking, and integration. Metabolites were annotated using public and proprietary databases, resulting in the final metabolite peak area tables.

Institutions

• Jiangnan University

  Jiangsu, Wuxi

Categories

Licence