Micro-CT and histological imaging dataset: guinea pig cochlear and otic capsule development (GD22–P5)

Description

This dataset contains micro-computed tomography (micro-CT) images, hematoxylin and eosin (H&E)-stained histological images, and gray-value measurements supporting the study “An Atlas of Cochlear Development and Otic Capsule Ossification in the Guinea Pig: A Combined Histological and Micro-CT Analysis from Early Gestation to the Early Postnatal Period” (Hearing Research, submitted). Guinea pig fetuses and neonates were examined at gestational day (GD) 22, 27, 32, 39, 47, 53 and postnatal day (P) 5. Micro-CT was acquired with a CosmoScan FX system (Rigaku) under stage-specific conditions (50 or 90 kV, 88 μA, 10–72 mm field of view, 20–144 μm voxel size). Histological sections were 5 μm thick, sampled at 50-μm intervals.

Steps to reproduce

Micro-CT images were analyzed in Fiji (ImageJ). Multiplanar reconstruction was used to generate axial images, and the mean gray value was measured within a manually placed region of interest on the cochlear portion of the bony labyrinth at the level of the basal turn, bilaterally in each specimen. See the Materials and Methods of the paper for details.

Institutions

• Nara Medical University

  Nara, Kashihara-shi

Categories

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