Ultrastructural characteristics of oligodendrocyte precursor cells in the early postnatal mouse optic nerve observed by serial block-face scanning electron microscopy.

Description

I upload the SBF-SEM images of newborn mouse optic nerve with 13 separate folders that are grouped into 4 datasets. I used these data for analyzing the glial cell morphology, especially focused on oligodendrocyte precursor cells. The results of analysis are accepted for publication by PLOS one. Purpose and results are as follows: Oligodendrocyte precursor cells (OPC) arise from restricted regions of the central nervous system (CNS) and differentiate into myelin-forming cells after migration, but their ultrastructural characteristics have not been fully elucidated. This study examined the three-dimensional ultrastructure of OPCs in comparison with other glial cells in the early postnatal optic nerve by serial block-face scanning electron microscopy. We examined 70 putative OPCs (pOPC) that were distinct from other glial cells according to established morphological criteria. The pOPCs were unipolar in shape with relatively few processes, and their Golgi apparatus were localized in the perinuclear region with a single cisterna. Astrocytes abundant in the optic nerve were distinct from pOPCs and had a greater number of processes and more complicated Golgi apparatus morphology. All pOPCs and astrocytes contained a pair of centrioles (basal bodies). Among them, 45% of pOPCs extended a short cilium, and 20% of pOPCs had centrioles accompanied by vesicles, whereas all astrocytes with basal bodies had cilia with invaginated ciliary pockets. These results suggest that the fine structures of pOPCs during the developing and immature stages may account for their distinct behavior. Additionally, the vesicular transport of the centrioles, along with a short cilium length, suggests active ciliogenesis in pOPCs.

Steps to reproduce

These images can be observed by the image-J software. If you would like to create 3D-reconstruction images from these data set, you can manage the images with Microscopy Image Browser (http://mib.helsinki.fi/) together with Amira software (Thermo Fischer Scientifics). folders 170809-ROI-00-1~170809-ROI-00-3 contain a series of images. folders 170809-ROI-01-1~170809-ROI-01-3 contain a series of images. folders 170809-ROI-02-1~170809-ROI-02-3 contain a series of images. folders 191202_ROI-4-1~191202_ROI-4-4 contain a series of images

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