BAF60a governs β-cell identity to control systemic glucose homeostasis
Description
In this study, we employed multiple sequencing techniques to investigate the role of BAF60a in maintaining systemic glucose homeostasis. This dataset comprises processed RNA-Seq, scRNA-Seq, and ATAC-Seq data from islets of flox/flox and β-cell specific BAF60a knockout (BaBKO) mice, revealing alterations in chromatin configuration, transcriptional networks, cell identity, and cellular metabolism resulting from BAF60a loss-of-function. Additionally, we provide a CUT&Tag-Seq dataset showing BAF60a binding sites on chromatin in chow and high-fat diet (HFD) fed mouse islets, along with the BAF60a interactome study shown in the manuscript. Detailed procedures for sample collection and data analysis are included in the Experimental Section of the manuscript.
Files
Steps to reproduce
RNA-Seq: core_table.csv: Islet RNA-Seq data from flox/flox and BaBKO mice, in 2 technical replicates. Islets were pooled from at least 3 individual mice. ScRNA-Seq: CTR_dge.txt.gz and BaBKO_dge.txt.gz: Digital Expression Matrix (dge) of islet scRNA-Seq from the flox/flox and BaBKO groups. Islets were pooled from at least 3 individual mice. ATAC-Seq: Pdx1CreCTR.bw and Pdx1CreKO.bw: BigWig files of islet ATAC-Seq from the flox/flox and BaBKO groups. Islets were pooled from at least 3 individual mice. CUT&Tag-Seq: BAF60a binding site folder: BigWig files of islet BAF60a CUT&Tag-Seq from mice fed with chow diet or HFD for 6 months, in 2 technical replicates. Islets were pooled from at least 3 individual mice. Chow diet samples: Chow_rep1_Coverage_noblacklist.bw, Chow_rep2_Coverage_noblacklist.bw. HFD samples: HFD_rep1_Coverage_noblacklist.bw, HFD_rep2_Coverage_noblacklist.bw. Proteomics: Proteomics.xlsx: Proteomics data of control (CTR) and BirA*-BAF60a interactome in Min6 cells.
Institutions
- Zhejiang University