Gas Chromatography and Mass Spectroscopy Based Detection of Lipid Profile in Jatropha Crude Oil and its Potential as Biodiesel Feedstock
Description
Jatropha curcas is one of the most suitable plants which seeds are non-edible in nature but rich in oil. In this research, the fatty acid composition of the extracted lipid of jatropha was studied using the gas chromatography with high resolution mass spectrometer. The major fatty acids in jatropha crude oil were the oleic (3.81%), linoleic (50%) and palmitic (35.66%) fatty acid. Some physical and chemical characteristics have been evaluated and found suitable for the application in engine. Oxidation stability oxidizability and cetane number has been calculated as 4.949, 1.076 and found 55.856. Iodine number correlates with oxidation stability, therefore iodine number is also considered as an index of oxidation stability. Standard iodine value for biodiesel was 120 for Europe’s EN 14214 specification while for Jatropha oil it was 103.62g I2/100g. This endeavor evaluates chemical and physical perspective of Jatropha crude oil and its suitability in internal combustion engine. 2 Material & Method- A gas-chromatograph with high resolution mass spectrometer was used to determine the free fatty acid composition of the Jatropha oil sample. The column length, diameter and thickness were 30m, 0.25mm and 0.25µm respectively. The conditions for analysis were set as follows; column oven temperature was programmed from 35°C-240°C (temperature at 35°C was held for 5min, raised to 210°C at 3°C/min and then finally to 240°C at 40°C/min and held for 4min); Helium gas was used as carrier gas, column flow of 1.80 mL/min for the GC. The injection volume was 2 μL with injection temperature of 250°C and a column flow of 1.80 mL/min for the GC. For the mass spectroscopy ACQ mode scanner with scan range of 30-700 amu at the speed of 1478 was used. The mass spectra were compared with the NIST05 mass spectral library ((National Institute of Standard and Technology, Gaithersburg) mass spectral library version 2). Result- Enclosed