Raw and processed sequence data for Sargassum polycystum-associated Trichoderma sp. SAR-C1-1 from Sayang Heulang Beach, Indonesia

Description

This dataset contains raw and processed Sanger sequencing data supporting the characterization of Trichoderma sp. SAR-C1-1 associated with Sargassum polycystum collected from Sayang Heulang Beach, Garut Regency, West Java, Indonesia. The dataset includes ITS and partial TEF1-α sequence data, sequence consensus files, BLAST-related data, TEF1-α phylogenetic analysis files, and associated metadata.The primary ITS sequence is the 591-bp consensus generated through Genetika Science Indonesia and deposited in GenBank under accession PZ950042. The partial TEF1-α consensus is 303 bp. The ITS and partial TEF1-α consensus sequences are publicly available in GenBank under accession numbers PZ950042 and QB030432, respectively. The dataset also includes the corresponding raw TEF1-α Sanger chromatograms, sequence files, BLAST-related data, phylogenetic analysis files, and associated metadata.

Steps to reproduce

Raw Sanger sequence reads were quality assessed and assembled into consensus sequences. The primary ITS consensus sequence was compared with NCBI GenBank records using BLASTn. TEF1-α forward and reverse Sanger reads were quality checked, primer-trimmed and assembled into a consensus sequence. Homologous TEF1-α sequences were aligned using MAFFT and maximum-likelihood phylogenetic inference was performed using IQ-TREE with ModelFinder and 5,000 ultrafast bootstrap replicates. The accompanying README describes the relationship between raw, processed and phylogenetic data files.

Institutions

• Padjadjaran University

  West Java, Bandung

Categories

Licence