Efficient Utilization of Compound Oligosaccharides by Lactobacillus paracasei ProSci-92 and Their Application in Fermented Milk

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The three files linked herein serve to present the supplementary materials for the manuscript.

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We adopted the Box–Behnken design to optimize oligosaccharide levels in probiotics due to its superior precision and greater experimental efficiency. Trehalose, inulin, and fructo-oligosaccharide were designated as independent variables, and probiotic growth performance, quantified as OD600, was selected as the response variable. The experimental design and oligosaccharide levels are listed in Supplemental Table S1. Data analysis was performed using the Design-Expert software

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