Trace Element Composition and DSC Data of Dental Tissues from Diabetic Patients
Description
This dataset contains raw and processed Differential Scanning Calorimetry (DSC) data obtained from dental tissue samples collected from type 2 diabetic and non-diabetic participants. Measurements were performed using a Shimadzu DSC-60 Plus calorimeter in the 25–600 °C range at a 2 °C/min heating rate. Data include temperature-enthalpy profiles (in mW vs °C) for enamel, dentin, and cementum regions. The dataset supports the article “Impact of Type 2 Diabetes on Dental Tissue Composition and Thermo-Structural Integrity. This dataset also includes quantitative elemental analysis results for dental tissue samples from type 2 diabetic and healthy participants, obtained by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). It provides concentrations (mg/kg) of Se, Zn, Cu, Cr, and Mg for crown and root regions of each sample group. All measurements were performed under identical analytical conditions using a Perkin Elmer Optima 8000 ICP-OES system. The dataset supports statistical comparisons reported in “Impact of Type 2 Diabetes on Dental Tissue Composition and Thermo-Structural Integrity.
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Steps to reproduce
Dental tissue samples (enamel, dentin, and cementum) were obtained from extracted teeth of healthy and type 2 diabetic adult patients. The tissues were cleaned, dried, and sectioned into crown and root portions. Each sample (~20–30 mg) was analyzed using a Shimadzu DSC-60 Plus Differential Scanning Calorimeter. The temperature range was 25–600 °C, with a heating rate of 2 °C/min under a nitrogen atmosphere. Both reference and sample pans were aluminum. Each run produced a heat flow (mW) versus temperature (°C) curve. Data were processed with Shimadzu TA-60 software, and enthalpy (ΔH) values were calculated using integrated peak areas. The method follows standard DSC protocols for biological and mineralized tissues. Samples were equilibrated to room temperature before measurement. Dental samples were mechanically cleaned, dried, and divided into crown and root regions. Each sample was digested using HNO₃–H₂O₂ (3:1 v/v) in Teflon tubes with microwave-assisted digestion at 180 °C for 30 min. The digested solutions were diluted with ultrapure water and analyzed using a PerkinElmer Optima 8000 ICP-OES instrument. Calibration curves were prepared from certified standard solutions (1000 mg/L stock for Se, Zn, Cu, Cr, and Mg). Data were collected using Syngistix software, with three replicate readings per element. Results are presented in mg/kg dry weight. All glassware and tubes were acid-washed, blanks were subtracted, and control standards were run every 10 samples. Detection limits and linearity were validated according to manufacturer guidelines.
Institutions
- Bahcesehir Universitesi Tip Fakultesi