Genome-wide CRISPR-Cas9 screens expose genetic vulnerabilities and mechanisms of TMZ sensitivity in glioblastoma stem cells. MacLeod and Bozek et al 2019.

Name: Genome-wide CRISPR-Cas9 screens expose genetic vulnerabilities and mechanisms of TMZ sensitivity in glioblastoma stem cells. MacLeod and Bozek et al 2019.
Creator: Graham MacLeod
Published: 2019-01-21T17:10:06.700Z
Keywords: Western Blot

MacLeod, Graham

doi:10.17632/tbrxk378kh.1

Genome-wide CRISPR-Cas9 screens expose genetic vulnerabilities and mechanisms of TMZ sensitivity in glioblastoma stem cells. MacLeod and Bozek et al 2019.

Published: 21 January 2019| Version 1 | DOI: 10.17632/tbrxk378kh.1

Contributor:

Graham MacLeod

Description

Representative western blot analysis of total protein lysate from G510NS-Cas9, G523NS-Cas9 and HF7450-Cas9 cells expressing the indicated gRNAs. Uncropped images used in the creation of Figure S3E.

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Antibodies Used: SOX2 (14-9811-82, Invitrogen), SOX9 (AB5355, Millipore Sigma), 𝛃-Tubulin (E7, University of Iowa Developmental Studies Hybridoma Bank)

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University of Toronto Leslie Dan Faculty of Pharmacy

Genome-wide CRISPR-Cas9 screens expose genetic vulnerabilities and mechanisms of TMZ sensitivity in glioblastoma stem cells. MacLeod and Bozek et al 2019.

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