Genome-wide CRISPR-Cas9 screens expose genetic vulnerabilities and mechanisms of TMZ sensitivity in glioblastoma stem cells. MacLeod and Bozek et al 2019.
Representative western blot analysis of total protein lysate from G510NS-Cas9, G523NS-Cas9 and HF7450-Cas9 cells expressing the indicated gRNAs. Uncropped images used in the creation of Figure S3E.
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Antibodies Used: SOX2 (14-9811-82, Invitrogen), SOX9 (AB5355, Millipore Sigma), 𝛃-Tubulin (E7, University of Iowa Developmental Studies Hybridoma Bank)