original data for the manuscript: Reprogramming orthogonal regulatory and metabolic system for secondary metabolite production

Published: 24 February 2026| Version 4 | DOI: 10.17632/th5mryvvmz.4
Contributors:
zhengduo wang, Haihong Chen, Yingrui Hou,
, Lingzhuo Zeng, Xi Li, Zilong Li, Baoxin Zhang, Lu Zhou, Lijie Niu, Binqing Yuan, Huan Zhu, Shanshan Li, Menglei Xia, Xiaohan Yang, Zhiheng Yang, Bixiao Li, Weilin Li, Hao Yan, Chengyu Zhang, Zonglin Liang, Lixin Zhang, Weishan Wang

Description

This dataset corresponds to the raw experimental data for the manuscript "Reprogramming orthogonal regulatory and metabolic system for efficient erythromycin production". It includes: 1. “Figure 2.xlsx”：Raw data supporting the "Development of orthogonal artificial control system" chapter in the main text. 2. “Figure 3.xlsx”：Raw data supporting the "Orthogonal PCoA and MMCoA supply module" chapter in the main text. 3. “Figure 4.xlsx”：Raw data supporting the "Fine-tuning the stoichiometry of precursors guided by in vivo kinetics" chapter in the main text. 4. “Figure 5.xlsx”：Raw data supporting the "Orthogonal methyl donor module" chapter in the main text. 5. “Figure 6.xlsx”：Raw data supporting the "Orthogonal desosamine and mycarose supply module" chapter in the main text. 6. “Figure 7.xlsx”：Raw data supporting the "Scale-up of the final strain with reprogrammed orthogonal regulatory and metabolic system" and the "Broad application of reprogramming orthogonal regulatory and metabolic system" chapters in the main text. 7. “Sup Figure.xlsx”：Raw data supporting the Supplementary Figures corresponding to this manuscript

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Steps to reproduce

Based on the detailed procedures and parameters provided in the Methods Details section of this manuscript, the original data submitted in this study can be reproduced.

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Categories

Bioengineering, Biotechnology, Microorganism-Derived Secondary Metabolite, Synthetic Biology, Actinomycete

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