Microarray Data for Rice
Description
NCBI Gene Expression Omnibus GEO (https://www.ncbi.nlm.nih.gov/geo/) Repository name: NCBI BioProject PRJNA681396 Data identification number: GEO accession GSE162302 www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE162302 GSM4948814 Prasanna_Seedlings-Shoot_0_1 [NMA1] GSM4948815 Prasanna_Seedlings-Shoot_0_2 [NMA2] GSM4948816 Prasanna_Seedlings-Shoot_100_1 [NMA3] GSM4948817 Prasanna_Seedlings-Shoot_100_2 [NMA4] GSM4948818 Varadhan_Seedlings-Shoot_0_1 [NMA5] GSM4948819 Varadhan_Seedlings-Shoot_0_2 [NMA6] GSM4948820 Varadhan_Seedlings-Shoot_100_1 [NMA7] GSM4948821 Varadhan_Seedlings-Shoot_100_2 [NMA8] GEO accession GSE162412 www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE162412 GSM4951206 Prasanna_Seedlings-Root_0 [NMA9] GSM4951207 Prasanna_Seedlings-Root_100_1[NMA11] GSM4951208 Prasanna_Seedlings-Root_100_2 [NMA12] GSM4951209 Varadhan_Seedlings-Root_0 [NMA14] GSM4951210 Varadhan_Seedlings-Root_100_1[NMA15] GSM4951211 Varadhan_Seedlings-Root_100_2 [NMA16]
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The rice genotypes Varadhan (efficient) and Prasanna (non-efficient) used in the present study were selected based on their contrasting NUE response. The seeds of Varadhan and Prasanna were surface sterilized with 1% sodium hypochlorite and germinated in Petri plates lined with wet filter paper under dark conditions. After two days, the germinated seedlings were transferred to the plant growth chamber and maintained for 10 days (16 hours light/ 8 hours dark, 24°C). The 12-day-old uniform seedlings were transferred to trays containing Yoshida nutrient solution following the randomized complete block design with two replications. The two hydroponics treatments viz., Yoshida nutrient solution with recommended N source (N+) and without N source (NH4NO3) (N-) were maintained at standard growth conditions in the plant growth chamber. The hydroponics nutrient solution was renewed every seven days. The shoot and root samples were harvested on the 34th day for morphological and physiological observations and also for RNA isolation for gene expression analysis through microarray.