DNA sequences of Neosilba and Lonchaea
Description
This dataset contains DNA sequences in FASTA format associated with the article "Avocado Sustains a Complex of Neosilba spp. (Diptera: Lonchaeidae) in Veracruz, Mexico" to be published. It includes molecular data for Neosilba and Lonchaea species analyzed in the study.
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Steps to reproduce
DNA was extracted from Neosilba spp. and Lonchaea cristula specimens. The COI gene was amplified using primers C1-J-1718 and C1-N-2191. Sequences were assembled and edited in BioEdit v7.0.5 software and aligned using MAFFT v7 and MEGA 7. The best nucleotide substitution model (GTR+I+G) was identified with jModelTest 2. Phylogenetic analyses were conducted using maximum likelihood in raxmlGUI 2.0 and Bayesian inference in MrBayes v3.2.6. The phylogenetic tree was edited in FigTree v1.4.3.