Processed transcriptomic and proteomic datasets supporting the multi-omics analysis in the manuscript
Description
This dataset contains processed transcriptomic and proteomic data generated from inguinal white adipose tissue collected from control mice, obesity-associated PCOS model mice, and PCOS mice treated with high-dose Atractylodis Rhizoma-derived exosome-like nanovesicles (AR-ELNs). These data support the multi-omics analyses presented in the associated manuscript and were used to characterize adipose metabolic-endocrine remodeling, with particular focus on kynurenine pathway activation and AhR/AR-related gene and protein programs in obesity-associated PCOS. The dataset includes processed RNA-seq differential expression tables and quantitative proteomics differential expression tables for the following comparisons: PCOS_vs_CTRL_iWAT_RNAseq_DEG_all_genes.xlsx Gene-level differential expression results comparing inguinal white adipose tissue from obesity-associated PCOS model mice with control mice. PCOS_H-ELNs_vs_PCOS_iWAT_RNAseq_DEG_all_genes.xlsx Gene-level differential expression results comparing inguinal white adipose tissue from PCOS mice treated with high-dose AR-ELNs with untreated PCOS model mice. PCOS_vs_CTRL_iWAT_Proteomics_DEP_all_proteins.xlsx Protein-level differential expression results comparing inguinal white adipose tissue from obesity-associated PCOS model mice with control mice. PCOS_H-ELNs_vs_PCOS_iWAT_Proteomics_DEP_all_proteins.xlsx Protein-level differential expression results comparing inguinal white adipose tissue from PCOS mice treated with high-dose AR-ELNs with untreated PCOS model mice. These processed datasets include gene- or protein-level identifiers, expression or abundance changes, fold-change values, statistical significance values, and annotation fields used for downstream pathway enrichment, integrated transcriptomic-proteomic analysis, and candidate pathway selection. The data are provided to support transparency and reproducibility of the multi-omics analyses reported in the manuscript.
Files
Steps to reproduce
The dataset contains processed transcriptomic and proteomic results used for the multi-omics analyses in the associated manuscript. Inguinal white adipose tissue samples were collected from control mice, obesity-associated PCOS model mice, and PCOS mice treated with high-dose Atractylodis Rhizoma-derived exosome-like nanovesicles (AR-ELNs). RNA-seq and quantitative proteomic analyses were performed on the same experimental groups. Differential expression analyses were conducted for PCOS versus control and PCOS + H-ELNs versus PCOS comparisons. The resulting gene- and protein-level tables were used for downstream pathway enrichment, integrated transcriptomic-proteomic analysis, and candidate pathway selection related to kynurenine pathway activation and AhR/AR-associated adipose remodeling. Users can reproduce the downstream analyses by importing the provided differential expression tables into R or other statistical software, filtering genes or proteins according to the thresholds described in the associated manuscript, and performing pathway enrichment or integrated omics analysis using the gene symbols, protein identifiers, fold-change values, and statistical significance values provided in each file.
Institutions
- Shanghai Traditional Chinese Medicine HospitalShanghai, Shanghai