Raw Figure from Single-cell MultiOmics and spatial transcriptomics demonstrate neuroblastoma developmental plasticity
Description
Raw blots shown in research article "Single-cell MultiOmics and spatial transcriptomics demonstrate neuroblastoma developmental plasticity"
Files
Steps to reproduce
Protein was extracted from the cells using 1% SDS lysis buffer (62.5mM Tris-HCl, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue) supplemented with Protease inhibitor cocktail (Beyotime, Shanghai, People’s Republic of China, ×100), resolved by SDS-polyacrylamide gels and then transferred to PVDF membranes (0.45 μm, Millipore, Billerica, MA, USA). Primary antibodies against E2F7 (0.5 μg/ml, Abcam), and GAPDH (1:10,000, Proteintech, China) were used. Horseradish peroxidase (HRP) conjugated secondary antibody (Anti-rabbit IgG, Anti-mouse IgG, Cell Signaling Technology) was used. Finally, the antigen-antibody reaction was visualized by the enhanced Pierce ECL Western blotting substrate kit (Thermo Scientific/Pierce, Rockford, IL, USA).