Microbial sequencing data (SLHA-treated tea plantation)

Published: 23 August 2026| Version 1 | DOI: 10.17632/vbt8wktx4h.1
Contributor:
Li Li

Description

Soil samples were collected from a tea plantation two years after the application of liquid derived from sulfite amine-cooked bamboo residues. The composition of the soil microbial community was assessed using high-throughput sequencing. Specifically, the 16S rRNA gene (primers 27F-1492R) and the Internal Transcribed Spacer (ITS) region (primers ITS1F-ITS4R) were amplified and sequenced.

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The full-length bacterial 16S rRNA gene and fungal ITS region were amplified using 27F/1492R and ITS1/ITS4, respectively, and sequenced using PacBio SMRT sequencing. Raw reads were processed using CCS for consensus correction, followed by quality filtering and chimera removal. High-quality sequences were clustered into operational taxonomic units (OTUs) at 98.5% sequence similarity and taxonomically assigned against the SILVA 138 and UNITE 9.0 databases for bacteria and fungi, respectively

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Microbiome, Soil, Agricultural Health, Bioconversion of Lignocellulosic Biomass, Agricultural Soil

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