The Diagnostic Value of Fecal Calprotectin for Positive Colonoscopic Findings in Chronic Diarrhea

Description

This is the raw data of an article titled 'The Diagnostic Value of Fecal Calprotectin for Positive Colonoscopic Findings in Chronic Diarrhea'. It includes the demographic characteristics of patients with chronic diarrhea (such as age, gender, BMI, etc.), diarrhea-related symptoms, endoscopic findings from colonoscopy, and fecal calprotectin levels. It also includes the relevant conditions of non-diarrhea controls.

Steps to reproduce

1.Clinical Data Collection Clinical data were systematically recorded for all individuals who consented to participate in the study during the initial screening phase, before the administration of bowel-cleansing agents, from all enrolled participants after informed consent was obtained. The dataset encompassed the following domains: 1) demographic characteristics (e.g., age, sex, and BMI); 2) disease-related symptoms, such as diarrhea, abdominal pain, and alarm symptoms (bloody stools, unintentional weight loss, fever and nocturnal diarrhea); 3) lifestyle factors, specifically current smoking status and alcohol consumption; and 4) common comorbidities (hypertension, diabetes, hyperlipidemia). 2.Stool Collection and Rapid Fecal Calprotectin Test Participants collected fecal samples within 24 hours of their scheduled colonoscopy, before ingestion of bowel-cleansing agents. To avoid contamination from toilet water, disposable cardboard stool collection devices were provided. Participants were instructed to transfer the stool into pre-sterilized, airtight plastic containers and transport them to the hospital on the day of the endoscopic procedure. Consequently, all samples were stored at room temperature for approximately 12 to 36 hours before analysis. Upon arrival at the hospital, the samples were immediately subjected to FC testing. FC concentration was quantified using the CGICA kit (Xiamen Wiz Biotech Co., Ltd., China). The testing procedure was as follows: a specific amount of fecal sample was first collected using a spiral-shaped sampling stick. The stick was then immersed in a tube containing the extraction buffer and shaken vigorously. Briefly, three drops of the mixture sample were loaded into the sample well on the cartridge using the tip on the tube. The cartridge was then inserted into the WIZ-A202 analyzer for reading. The instrument automatically scans the signal intensities of the test (T) and control (C) lines, and the calprotectin concentration (in µg/g) is calculated and reported using a built-in standard curve. Each assay included a quality control step, and results were considered valid only if the control line was typically developed. The detection range of this method is 10-2500 µg/g. Throughout the study period, the gastroenterologists who established the final diagnoses were blinded to the FC results.

Institutions

• Wuhan Union Hospital

  Hubei, Wuhan
• Huazhong University of Science and Technology Tongji Medical College

  Hubei, Wuhan

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