Effect of temperature on hematological and biochemical parameters when the biological sample from rats kept in room temperature, thermocol, polypropylene box and innovative portable refrigerator
Description
This data was generated for the preclinical studies where biological samples from rats such as blood, serum, liver were stored in different storage conditions such as room temperature, thermocol box, polypropylene box and in an innovative portable battery-operated re-chargeable refrigerator "Emvolio" developed by Blackfrog Technologies Private Limited, Manipal, India. The integrity of biochemical and hematological parameters are given, which indicates that Emvolio is maintaining a constant temperature for 10 hrs. The data also suggests that Emvolio can be effectively used in preclinical sample preservation and transportation. Emvolio is battery operated small, volume refrigerator for medicines, vaccines, food, beverages, dairy etc. and has Indian Patent (Patent number 201941013056; https://ipindiaservices.gov.in/publicsearch). These data also support its preclinical uses in laboratories where animal test samples can be preserved and transported.
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Steps to reproduce
The main data was generated from the animal experimentation where the institutional ethical committee approval was obtained (#IAEC/KMC/81/2019), and followed all the guidelines of CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals). Different biological samples such as blood serum and liver were obtained after humanely killing the rats under anaesthesia. These samples were then preserved and stored at room temperature, thermocol box, polypropylene box and Emvolio for more than six hours, and the temperature was maintained. For each 0, 3 and 6 hrs, the hematological parameters and the biochemical parameters were determined. For the enzyme assays, standard autoanalyzer kits were used. The test was done in triplicate to avoid errors. Parameters tested and compared are blood parameters, namely Red Blood Cells (RBC), White Blood Cells (WBC), Platelets, Haematocrit, Haemoglobin, Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin Concentration (MCHC) and Red Cell Distribution Width (RDW), serum parameters like Alanine Transaminase (ALT), Alkaline Phosphatase (ALP), Total Protein, Albumin, Creatine, Blood Urea Nitrogen and liver parameters like Superoxide Dismutase (SOD), Glutathione (GSH), Catalase.