Loss of Histone Methyltransferase Ezh2 Exacerbates Polarization of Macrophages toward M2-Like Phenotypes by Hepatocellular Carcinoma

Description

The raw, unadjusted, uncropped Western blot with size markers for the Western blot data

Steps to reproduce

Total protein of BMDM was harvested by using RIPA lysis buffer and subjected to SDS-PAGE and Western blot. Antibodies were diluted in 0.05% Tween in PBS with 3% (w/v) skim milk or 0.1% Tween in TBS with 5% (w/v) bovine serum albumin (Sigma-Aldrich). The signal was detected by the ECL chemiluminescent detection method. Relative intensity was analyzed by ImageJ software (Version 1.54g). The antibodies used in this study are mouse anti-β-Actin (Cat. 3700), rabbit anti-Stat3 (Cat. 9132), rabbit anti-p-Stat3 (Tyr705) (Cat. 9131), rabbit anti-Arginase I (Cat. 93668), rabbit anti-iNOS (Cat. 2977), goat anti-rabbit IgG, HRP-linked antibody (Cat. 7074), rabbit anti-PU.1 (Cat. 2258). All reagents were from Cell Signaling Technology. Sheep anti-mouse IgG, HRP-linked antibody (NA9310-1ML) from Cytiva.

Institutions

• Chulalongkorn University

Categories

Funders

• The NSRF via the Program Management Unit for Human Re¬sources & Institutional Development, Research and Innovation

  Grant ID: B16F640117 and B13F670075
• Thailand Science Research and Innovation Fund Chulalongkorn University

  Grant ID: FF68
• National Research Council of Thailand (NRCT)

  Grant ID: N42A680063

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