Genome-wide Brie CAD5 Surface PrPC - Differentiated PrPC High

Published: 13 June 2025| Version 1 | DOI: 10.17632/vs9fwhbcvj.1
Contributor:
Kathryn Beauchemin

Description

These are the raw .fastq files from NGS runs of the genome-wide CRISPR/Cas9 KO library (Brie) performed in differentiated CAD5 cells. They are labeled Brie_PrPC_High to indicate that these sgRNA sequences were captured in the "PrPC High" populations during FACS. Bioinformatic analysis was performed using the MAGeCK-VISPR and MAGeCKFlute pipelines using the biological replicates function to compare between "PrP Low" and "PrP High" samples.

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Institutions

  • Dartmouth College

Categories

Prion, CRISPR

Funders

Licence