The Diagonal Technique for Alopecia Biopsies

Published: 7 May 2021| Version 1 | DOI: 10.17632/vvp4px3scf.1
Contributors:
Cynthia Harris, Daniel Shepherd, Denise Bland, William Anim, Aliyah Sohani, Lyn Duncan, Rosalynn Nazarian

Description

Supplementary Figure 1. Scarring Alopecia. A representative scalp biopsy processed using the diagonal technique demonstrates a change in tissue contour from circular (A, 2x, H&E) to ovoid (B, 2x, H&E) for the superficial half of the punch biopsy, angled at time of embedding. The isthmus is visualized in an intermediate level (C, 4x, H&E). Elastic stain demonstrates loss of the perifollicular elastic network with adjacent elastic fiber thickening and recoil (D, 40x, elastic stain) supporting the diagnosis of scarring alopecia.

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Institutions

  • Massachusetts General Hospital

Categories

Dermatology, Dermatopathology, Alopecia, Quality Improvement, Labor Management

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