Date (CATENA)
Description
(1) study the growth and Cd and As uptake and accumulation of hyperaccumulators when intercropped with R. pseudoacacia L.; (2) explore the influence of R. pseudoacacia L. intercropped with hyperaccumulators on the fundamental physicochemical characteristics of soil and soil enzymes activities; (3) evaluate the response of N-fixing microbial community and co-occurrence networks under the intercropping of R. pseudoacacia L. and hyperaccumulator; (4) investigate the relationships between the uptake of Cd and As in hyperaccumulators and the soil N-fixing microbial communities under the intercropping system.
Files
Steps to reproduce
10.0 kg of the tested soil (Soil L and Soil H) were added to a plastic pot (length × width × height: 30 × 25 × 30 cm). 0.28 g/kg CO(NH2)2, 0.21 g/kg (NH4)3PO4, and 0.22 g/kg K2CO3 were applied as base fertilizers, followed by the addition of deionized water to maintain 70% field capacity. After 7 d equilibrium, seedlings of R. pseudoacacia L., S. nigrum L., and P. vittata L. were transplanted into the plastic pots in accordance with the following experimental treatments: monoculture of R. pseudoacacia L., each pot contained 6 seedlings (R), monoculture of S. nigrum L., each pot contained 6 seedlings (S), monoculture of P. vittata L., each pot contained 6 seedlings (P), intercropping of R. pseudoacacia L. and S. nigrum L., each pot contained 3 seedlings of each plant (RS), intercropping of R. pseudoacacia L. and P. vittata L., each pot contained 3 seedlings of each plant (RP), and intercropping of R. pseudoacacia L., S. nigrum L., and P. vittata L., each pot contained 2 seedlings of each plant (RSP). A pot without any plants was considered as a control (CK). Three replicates were set for each treatment. Vigorous and uniform sized seedlings of R. pseudoacacia L. (15-20 cm in height), S. nigrum L. (8-12 cm in height), and P. vittata L. (10-15 cm in height) were utilized for the pot experiment. The pot experiment was under natural light conditions. During the entire cultivation period, deionized water was used for irrigation based on soil moisture levels. After 180 d of cultivation, plant samples were collected and divided into root, stem (rhizoid), and leaf (frond). Plant samples were first rinsed with tap water and subsequently washed thoroughly with pure water, then dried at 105℃ for 30 minutes followed by drying at 70℃ to a constant weight. Rhizosphere soil samples for each treatment were obtained by shaking plant roots and stored at -80℃ for soil DNA extraction. Meanwhile, bulk soil samples from each treatment were collected to analyze the key basic physicochemical properties, soil enzyme activities, available Cd/As levels, and nitrogen fractions in the soil.
Institutions
- Central South University of Forestry and Technology