Microorganism survival curve and food safety of irradiated honey
Description
the number of colonies in the irradiated samples was counted, and the results are shown in Data 1 and 2 in Data File . According to the results in Data 1 and 2 in Data File, it can be seen that the number of colonies decreases with the increase of the radiation dose until no colonies are seen at a dose of 15 kGy and above. Therefore, a dose of 15 kGy is the most appropriate dose for radiation. To determine the effect of irradiation on the physical and chemical properties of honey samples, results of the report Data 4 in Data File shows the properties of non-irradiated samples and Data 5 in Data File shows the properties of irradiated samples with 15 kGy dose. Data 5 shows the properties of honey in the most suitable dose (15 kGy) for sterilization. To investigate the effect of irradiation on the changes made in the chemical bonds of the irradiated honey samples, the FTIR transmission spectrum of the samples was obtained. Data 6 in Data File (SPSS file) shows the spectrum of the control sample and the irradiated samples with doses of 10 and 20 kGy. Statistical Analys: To understand the significant relationship between radiation doses and the reduction of the population of microorganisms in honey, we performed ANOVA on the data obtained from three times of cultivation of the control honey sample and the irradiated samples with different doses, the data of which are given in Data 1 in Data File and Data 2 in Data File. The results of this ANOVA are given in Data 7 in Data File. Also to quantitatively understand the effect of gamma radiation on the chemical environment of honey with increasing radiation dose, we calculated the correlation coefficient between the FTIR spectral data of samples S2, S3, S4, S5, S6 with the control sample S0 using SPSS software by the Spearman method and the results are shown in Data 8 in Data File.From the results of Data 7 in Data File, we see that irradiation up to a dose of 15 kGy has a coefficient of More than 0.995 was obtained between the entire wavenumber range of the spectrum of the irradiated samples and the control sample, which is almost constant with an accuracy of 0.005. However, with increasing the irradiation dose of the samples to 20 kGy, this coefficient decreased to 0.978, and for honey irradiation with a dose of 25 kGy, this coefficient decreased to 0.857, indicating that the effect of radiation on the chemical environment of honey at doses higher than 51 kGy is significant and that sterilization of honey up to a dose of 15 kGy does not destroy the food safety of irradiated honey.
Files
Institutions
- University of Mohaghegh Ardabili