HIPSC-Placenta_on-a-chip-iPlacenta
Published: 9 January 2023| Version 1 | DOI: 10.17632/wrxsr5drvy.1
Contributors:
Colin Murdoch, Description
RNA Seq data
Files
Steps to reproduce
Human induced pluripotent stem cells (hiPSC)-derived trophoblasts into a multi-well microfluidic device to develop a physiologically relevant and scalable placental barrier model. Cultured hiPSC-trophoblasts in 2D or in a perfused 3D micro-channel against a collagen-based matrix. Examined hiPSC-derived trophoblasts behaviour to self-arranged into a 3D structure showing invasive behaviour, fusogenic and endocrine activities, structural integrity and expressing placental transporters. Conducted RNAseq analysis to assesss expression between 2D and 3D, as well as differentation over period of time.
Institutions
University of Dundee School of Medicine
Categories
System on a Chip, Induced Pluripotent Stem Cell, Basic Science, In Vitro Techniques, Placental Perfusion in Vitro
Funding
H2020 Marie Skłodowska-Curie Actions
765274
Tenovus
T18-33
Seventh Framework Programme
626633