Data for: Comparisons of the pharmacokinetic and tissue distribution profilesof withanolide B after intragastric administration of the effective part of Datura metel L. in normal and psoriasis guinea pigs

Published: 30 Aug 2018 | Version 1 | DOI: 10.17632/x3vkrx9hcm.1

Description of this data

A simple, highly sensitive ultra-performance liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed to quantify of withanolide B and obakunone (IS) in guinea pig plasma and tissues, and to compare the pharmacokinetics and tissue distribution of withanolide B in normal and psoriasis guinea pigs. After mixing with IS, plasma and tissues were pretreated by protein precipitation with methanol. Chromatographic separation was performed on a C18 column using aqueous (0.1% formic acid) and acetonitrile (0.1% formic acid) solutions at 0.4 mL/min as the mobile phase. The gradient program was selected. Detection was performed on a 4000 QTRAP UPLC–ESI-MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. Withanolide B and obakunone (IS) were monitored under positive ionization conditions. The optimized mass transition ion-pairs (m/z) for quantitation were 455.1/109.4 for withanolide B and 455.1/161.1 for obakunone.

Experiment data files

This data is associated with the following publication:

Comparisons of the pharmacokinetic and tissue distribution profiles of withanolide B after intragastric administration of the effective part of Datura metel L. in normal and psoriasis guinea pigs

Published in: Journal of Chromatography B

Latest version

  • Version 1

    2018-08-30

    Published: 2018-08-30

    DOI: 10.17632/x3vkrx9hcm.1

    Cite this dataset

    Kuang, Haixue; Zhang, Lijie; Yang, Lianrong; Meng, Xin; Yang, Bingyou (2018), “Data for: Comparisons of the pharmacokinetic and tissue distribution profilesof withanolide B after intragastric administration of the effective part of Datura metel L. in normal and psoriasis guinea pigs”, Mendeley Data, v1 http://dx.doi.org/10.17632/x3vkrx9hcm.1

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Liquid Chromatography

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