Quorum sensing controls adaptive immunity through the regulation of multiple CRISPR-Cas systems

Published: 18 November 2016| Version 1 | DOI: 10.17632/x55v9zf59x.1
Contributors:
Adrian Patterson,
Simon Jackson,
Corinda Taylor,
Gary Evans,
George Salmond,
Rita Przybilski,
Raymond Staals,
Peter Fineran

Description

This is the original gel image for figure 4 and complementation images for Figure S4 in the "Quorum sensing controls adaptive immunity through the regulation of multiple CRISPR-Cas systems" manuscript.

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Steps to reproduce

CRISPR expansion (indicative of spacer acquisition) was determined by PCR directly on cells from overnight cultures (DreamTaq, Thermo Fisher Scientific) using primers PF1887 + PF1989 for CRISPR1 and PF1888 + PF1990 for CRISPR2. PCR products were separated by 3% agarose gel electrophoresis, stained with ethidium bromide and spacer acquisition was quantified using ImageJ (Schneider et al., 2012).

Institutions

University of Otago

Categories

Polymerase Chain Reaction, Gel

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