The expression changes of hepatic proteasome proteins across different feeding regimens
Description
Our transcriptional data showed that the expression of the hepatic genes encoding proteasome subunits switched at the time after fasting or refeeding for 16 hours. Since the amount of proteasomes in Mammalia is reported to be regulated at the transcriptional level, we suspect the expression of hepatic proteasome proteins will be sensitive to feeding behavior. To verify this hypothesis, we performed a Western blot analysis of three hepatic proteasome proteins (PSMB3, PSMA4, PSMB6) across different feeding regimens. The data confirmed our hypothesis. ACTB, actin beta; AF, acute fasting; AL, ad libitum; AR, refeeding after acute fasting; BAT, brown adipose tissue; EODF, every other day fasting; EODR, every other day refeeding; PSMA4, proteasome 20S subunit Alpha 4; PSMB3, proteasome 20S subunit beta 3; PSMB6, proteasome 20S subunit beta 6.
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Steps to reproduce
Western blot analysis for PSMB3, PSMA4, and PSMB6