Attenuating cardiac fibrosis and apoptosis by exercise is associated with modulation of the circSlc8a1/miR-133a axis in D-galactose-treated rats
Description
This dataset contains the raw experimental data supporting the study entitled “Attenuating cardiac fibrosis and apoptosis by exercise is associated with modulation of the circSlc8a1/miR-133a axis in D-galactose-treated rats.” The dataset was generated to investigate the effects of exercise on cardiac fibrosis and apoptosis and to examine the potential involvement of the circSlc8a1/miR-133a regulatory axis in D-galactose-treated rats. The deposited dataset includes raw real-time quantitative PCR (RT-qPCR) data and original Western blot images obtained from the experimental groups. The RT-qPCR data contain the original measurements used to evaluate the expression of circSlc8a1, miR-133a, and genes associated with cardiac fibrosis and apoptosis. These measurements provide the primary molecular data used to compare gene and non-coding RNA expression among the experimental groups and to support the analyses presented in the associated manuscript. The Western blot component of the dataset consists of the original experimental images obtained during the analysis of protein expression in cardiac tissue. These images represent the primary protein-level evidence used to investigate fibrosis- and apoptosis-related markers and to assess molecular changes associated with D-galactose treatment and exercise intervention. The original images are provided to support transparency and allow independent evaluation of the reported protein expression findings. Together, the deposited RT-qPCR and Western blot data provide complementary evidence at the RNA and protein levels. The RT-qPCR data support the assessment of the circSlc8a1/miR-133a axis and related molecular targets, while the Western blot images support the evaluation of proteins involved in cardiac fibrosis and apoptosis. The dataset therefore provides the underlying experimental measurements used to generate and interpret the molecular results reported in the study. The deposited files are intended to facilitate research transparency, reproducibility, and independent verification of the findings. Researchers may use the data to examine the reported expression patterns, conduct appropriate secondary analyses, and further investigate the molecular mechanisms associated with exercise-induced cardioprotection, cardiac remodeling, fibrosis, apoptosis, circular RNAs, and microRNAs. The data should be interpreted together with the associated manuscript, which provides detailed information regarding the experimental design, D-galactose treatment, exercise intervention, sample collection, molecular assays, normalization procedures, statistical analyses, and interpretation of the findings. The dataset does not replace the methodological information provided in the manuscript but provides access to the underlying experimental measurements supporting the reported results. By making these data available, the dataset contributes to the transparency and reproducibility of the reported findings and future work ahead.
Files
Institutions
- Kerman University of Medical SciencesKerman, Kerman