Supplementary Table 1. Three SNPs co-segregated with the Xwgc2315 STS locus on distal 7B; SNP map positions from Wang et al. (2014), STS marker details from Zhang et al. (2022).
Description
Three SNP polymorphisms co-segregated with the Xwgc2315 STS locus located at the distal end of chromosome 7B. The consensus map positions of the three SNP loci were provided by (S. Wang et al., 2014) and the STS marker detail were obtained from W. Zhang et al. (2022)
Files
Steps to reproduce
A set of 20 positive (Fhb7) and 14 negative controls, along with 60 BC1F1 plants, were genotyped using the STS marker WGC2315 and wheat 90K SNP data. Positive controls included the Th. elongatum/Chinese Spring (CS) amphiploid, the 7J addition line to CS, and the CS translocation line, as described by Zhang et al. (2022). PCR amplification of the STS marker WGC2315 produced a 941 bp amplicon in positive controls, while no amplification was observed in negative controls. For BC1F1 plants, DNA was extracted, PCR-amplified with the WGC2315 primer pair, and scored for presence/absence of the 941 bp band. In parallel, SNP genotype data were retrieved from the 90K SNP dataset, and loci mapped to chromosome 7B were filtered (Wang et al., 2014). Polymorphisms were compared between positive and negative controls to identify co-segregating SNP markers. Three such SNPs were consistently associated with the WGC2315 locus and mapped to the distal end of 7BL. Segregation in the BC1F1 population (30 positive : 30 negative) was consistent with monogenic inheritance of the Fhb7 translocation segment in bread wheat.