rps16-trnQ+ trnL-F cpDNA sequences from Pilea pteridophylla (Urticaceae)
Published: 9 August 2023| Version 1 | DOI: 10.17632/xxbkfcdnnc.1
Contributor:
Andrés Ernesto Ortiz-RodriguezDescription
A matrix of 462 bp for trnL-F and 870 bp for rps16-trnQ. A concatenated matrix (rps16-trnQ+ trnL-F) consisted of 1332 bp
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Steps to reproduce
For trnL-trnF amplifications, we used the universal primers (trnL-e, trnL-f) designed by Taberlet et al. (1991), whereas for the trnQ-rps16, we used the primers (trnQ-5’, rps16x1) described by Shaw et al. (2007). Protocols for DNA amplification by PCR assays and for sequencing the PCR products with some minor modifications as described elsewhere (Ornelas et al., 2016).
Institutions
Universidad Nacional Autonoma de Mexico
Categories
Genetics, Taxonomy, Phylogeny, Phylogeography