rps16-trnQ+ trnL-F cpDNA sequences from Pilea pteridophylla (Urticaceae)
A matrix of 462 bp for trnL-F and 870 bp for rps16-trnQ. A concatenated matrix (rps16-trnQ+ trnL-F) consisted of 1332 bp
Steps to reproduce
For trnL-trnF amplifications, we used the universal primers (trnL-e, trnL-f) designed by Taberlet et al. (1991), whereas for the trnQ-rps16, we used the primers (trnQ-5’, rps16x1) described by Shaw et al. (2007). Protocols for DNA amplification by PCR assays and for sequencing the PCR products with some minor modifications as described elsewhere (Ornelas et al., 2016).