HLA Profile of Kazakhs

Description

We analyzed HLA class I and II diversity in 2,712 unrelated healthy individuals of Kazakh descent typed at HLA-A, -B, -C, -DRB1, and -DQB1 loci. A total of 306 alleles were identified across the five loci. The most frequent alleles were HLA-A*02:01 (18.75%), B*51:01 (8.89%), C*06:02 (15.23%), DRB1*07:01 (13.09%), and DQB1*03:01 (23.65%). The most common two-locus haplotypes were A*33:03B*58:01 (2.38%), B*13:02DRB1*07:01 (4.38%), B*13:02C*03:04 (6.10%), and DRB1*03:01DQB1*02:01 (9.26%). The most frequent five-locus haplotypes were A*33:03C*03:02B*58:01DRB1*03:01DQB1*02:01 (1.51%) and A*01:01C*07:01B*08:01DRB1*03:01DQB1*02:01 (1.03%).

Steps to reproduce

Genomic DNA was extracted from anticoagulated peripheral blood using the FlexiGene DNA Kit (Qiagen, Hilden, Germany). HLA class I (A, B, C) and class II (DRB1, DQB1) loci were typed by PCR with sequence-specific priming using One Lambda SSP1L and SSP2L kits (One Lambda, Thousand Oaks, CA). Genotypes were assigned with HLA Fusion 4.0 software. Final allele assignments were reported at two-field resolution in accordance with the current IPD-IMGT/HLA nomenclature.

Institutions

• Tunis El Manar University

  Tunis Governorate, Tunis

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