EXT1-dependent lipidomics, glycomics, and proteomics

Description

Comparison between lipid species (Table S5), N- and O-glycans (Table S2), and peptides (Table S4) isolated from Endoplasmic reticulum membranes of EXT1 knock-down and control HeLa cells. Proteins identified by mass spectrometry, as partners of EXT1 in ER membranes (Table S6).

Steps to reproduce

1-Generate Hela cells knock-down for EXT1 and control cells 2-Isolate ER membranes 3-Isolate and quantify lipid species, glycans and peptides 4-For interacting partners, generate cells over expressing a Flag-tagged EXT1 protein 5-Isolate ER membranes 6-Immunoprecipitation of Flag-EXT1 7-Mass spectrometry analyses

Institutions

Categories

Licence