In vitro growth curve data of isolates exposed to inflammatory compounds
Description
The following folder contains individual prism files for each growth curve condition included in Sinha et al., (2025) Figure. 1. Each file contains OD600 readings (normalized to blank), growth curve plots, area under the curve calculations and, if necessary, virus-like particle quantification for each condition.
Files
Steps to reproduce
Four bacterial strains were grown anaerobically (Mandel Scientific Company Inc., Guelph, ON, Canada; 5 % Hydrogen, 20 % Carbon Dioxide, 95 % Nitrogen). A. muciniphila YL44, A. muciniphila BAA-835 and B. caecimuris I48 were grown in BHI supplemented with hemin (5 g/mL), vitamin K (1 g/mL) and 0.05 % L-cysteine. F. plautii YL31 was grown in AAM. Strains were grown overnight at 37°C and then sub-cultured to a starting OD600 = 0.1 (n=3). Subcultures were grown in 96-well plates with inflammatory compounds of interest (Table. 1) or the corresponding vehicle controls to reach a final volume of 200 uL. Bacterial growth was monitored by measuring the OD600 (Epoch 2 microplate spectrophotometer, Biotek Instruments) every 15 min, with orbital mixing until stationary phase. The time at which stationary phase was reached varied slightly between different strains and compounds. In the absence of inflammatory compounds, B. caecimuris (~ 8 hr), F. plautii (~9 hr) generally reached stationary quicker than the two A. muciniphila strains, YL44 (~ 24 hr) and BAA-835 (~ 22 hr). The AUC for each condition compared to its vehicle control was calculated using GraphPad Prism (v.8.4.3).